Thursday, June 27, 2013

Mouse cloned from drop of blood

Scientists in Japan have cloned a mouse from a single drop of blood.

Circulating blood cells collected from the tail of a donor mouse were used to produce the clone, a team at the Riken BioResource Center reports in the journal Biology of Reproduction.

The female mouse lived a normal lifespan and could give birth to young, say the researchers.

Scientists at a linked institute recently created nearly 600 exact genetic copies of one mouse.

Mice have been cloned from several different sources of donor cells, including white blood cells found in the lymph nodes, bone marrow and liver.

Continue reading the main story

?Start Quote

This technique would be applicable for generating genetic copies of invaluable strains of mice?

End Quote Research team Riken BioResource Center, Tsukuba

The Japanese research group investigated whether circulating blood cells could also be used for cloning.

Their aim was to find an easily available source of donor cells to clone scientifically valuable strains of laboratory mice.

The team, led by Atsuo Ogura, of Riken BioResource Center in Tsukuba, took blood from the tail of a donor mouse, isolated the white blood cells, and used the nuclei for cloning experiments, using the same technique that produced Dolly the sheep in Edinburgh.

The process, known as somatic cell nuclear transfer, involves transferring the nucleus from an adult body cell - such as a blood or skin cell - into an unfertilised egg that has had its nucleus removed.

Reporting their findings in the US journal, Biology of Reproduction, the scientists said the study "demonstrated for the first time that mice could be cloned using the nuclei of peripheral blood cells".

'Invaluable strains'

They added: "These cells could be used for cloning immediately after collection and no donor animals need to be euthanised.

Continue reading the main story

The cloning method - somatic cell nuclear transfer

  • Clones of adult animals are produced by a method called somatic cell nuclear transfer, which refers to the transfer of the nucleus from a somatic cell into an egg cell
  • A somatic cell is any cell of the body apart from a germ (sex) cell
  • In cloning, the nucleus of a somatic cell is removed and inserted into a donor unfertilised egg that has had its own nucleus (containing the genetic material) removed
  • The embryo is then placed inside a surrogate mother.

"This technique would be applicable for generating genetic copies of invaluable strains of mice, which cannot be preserved by other assisted reproductive techniques such as conventional in vitro fertilisation or intracytoplasmic sperm injection."

Scientists in Japan have years of experience in cloning mice.

A team at a linked institute recently revealed they had produced almost 600 mice from one donor mouse after 25 consecutive rounds of cloning.

The research is aimed at large-scale production of high-quality animals for farming or conservation purposes, they say.

Commenting on the study, Professor Robin Lovell-Badge of the MRC National Institute of Medical Research in London, said it was a useful small advance on previous work showing that it is possible to clone mice from a variety of adult cell types, including white blood cells.

He told BBC News: "The efficiency of cloning from these cell types was very good, suggesting that even a small drop of blood will contain sufficient numbers...this is helpful if the intention is to use cloning to propagate and expand numbers of rare or valuable types of individual or species."

Source: http://www.bbc.co.uk/news/health-23068423#sa-ns_mchannel=rss&ns_source=PublicRSS20-sa

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HTC may be the only One able to stop Samsung?s Android hegemony

Impertinent. Mumbling. Offended. Teary-eyed. Rachel Jeantel, star witness for the prosecution in George Zimmerman's murder trial, was all of those, and more, as her testimony Wednesday provided new details into Trayvon Martin?s last moments and infused racially loaded commentary into an already-sensitive trial.

Source: http://news.yahoo.com/htc-may-only-one-able-stop-samsung-android-205024980.html

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'Despicable Me's' Carell: 'I hope I'm a cool dad'

Movies

June 26, 2013 at 10:27 AM ET

There are times in your life when career changes just have to be made -- and Steve Carell, who left "The Office" to pursue a film career, knows all about that. And in "Despicable Me 2," he returns as former evildoer Gru, who has left the bad guy biz to look after three young girls and make "terrible" jams and jellies, as the actor explained to TODAY's Savannah Guthrie Wednesday.

"(Gru) needed to shake it up," said Carell. "He's sort of at a career impasse. He can't be a villain any more because he's got these three little girls to take care of now. He has a lot on his plate right now."

The funnyman said he actually empathized with some of what Gru is going through -- one of the daughters in the movie is hitting her teen years and finding an interest in boys, while in real life Carell says he's bracing for when those emotions well up in his real-life 12-year-old daughter.

"There's that anticipation of 'Am I going to be an overprotective dad?'" he wondered. "I will roll with it. I hope I'm a cool dad."

"Despicable Me 2" opens in theaters on July 3.

Source: http://www.today.com/entertainment/despicable-mes-steve-carell-i-hope-im-cool-dad-6C10455619

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Thursday, June 20, 2013

Obama's Berlin speech: History raises the stakes

U.S. President Barack Obama, left, is greeted by German President Joachim Gauck, right, during his arrival at Schloss Bellevue, in Berlin, Germany, Wednesday, June 19, 2013. Obama is on a 24-hour visit to Germany, the culmination of which will be a speech at Berlin's iconic Brandenburg Gate. (AP Photo/Pablo Martinez Monsivais)

U.S. President Barack Obama, left, is greeted by German President Joachim Gauck, right, during his arrival at Schloss Bellevue, in Berlin, Germany, Wednesday, June 19, 2013. Obama is on a 24-hour visit to Germany, the culmination of which will be a speech at Berlin's iconic Brandenburg Gate. (AP Photo/Pablo Martinez Monsivais)

U.S. President Barack Obama, second from right, is welcomed by German President Joachim Gauck at Schloss Bellevue on Wednesday, June 19, 2013, in Berlin. Obama will renew his call to reduce the world's nuclear stockpiles, including a proposed one-third reduction in U.S. and Russian arsenals, a senior administration official said. (AP Photo/Evan Vucci)

U.S. President Barack Obama, right, is welcomed by German President Joachim Gauck at Schloss Bellevue on Wednesday, June 19, 2013, in Berlin. Obama will renew his call to reduce the world's nuclear stockpiles, including a proposed one-third reduction in U.S. and Russian arsenals, a senior administration official said. (AP Photo/Evan Vucci)

US President Barack Obama and first lady Michelle Obama and their daughters Sasha, left, and Malia disembark from Air Force One at the Tegel airport in Berlin Tuesday, June 18, 2013. Obama arrived for a two-day official visit to Germany. (AP Photo/Michael Probst)

Onlookers take snapshots as the motorcade carrying United States President Barack Obama passes the Brandenburg Gate after arriving in Berlin, Germany, Tuesday, June 18, 2013. Obama arrived for a two-day official visit to Germany and will deliver a speech in front of the Brandenburg Gate Wednesday June 19, 2013. (AP Photo/Gero Breloer)

BERLIN (AP) ? Five years and 50 years. As President Barack Obama revisits Berlin, he can't escape those anniversaries and the inevitable comparisons to history and personal achievement.

With his own 2008 speech at Berlin's Victory Column and former President John F. Kennedy's 1963 historic denunciation of the Soviet bloc as markers, Obama will use an address at the city's Brandenburg Gate on Wednesday to renew his call to reduce the world's nuclear stockpiles.

The White House said Obama will draw attention to his plan for a one-third reduction in U.S. and Russian arsenals, rekindling a goal that was a centerpiece of his early first-term national security agenda. Obama will also hold an afternoon news conference with German Chancellor Angela Merkel after a meeting between the two leaders.

His 26-hour whirlwind visit to the German capital caps three days of international summitry for the president and marks his return to a place where he once summoned a throng of 200,000 to share his ambitious vision for American leadership.

That was 2008, when Obama was running for president and those who supported him at home and abroad saw the young mixed-race American as a unifying and transformational figure who signified hope and change.

Five years later, Obama comes to deliver a highly anticipated speech to a country that's a bit more sober about his aspirations and the extent of his successes, yet still eager to receive his attention at a time that many here feel that Europe, and Germany in particular, are no longer U.S. priorities. A Pew Research Center poll of Germans found that while their views of the U.S. have slipped since Obama's first year in office, he has managed to retain his popularity, with 88 percent of those surveyed approving of his foreign policies.

Obama also has an arc of history to fulfill.

Fifty years ago next week, President Kennedy addressed a crowd of 450,000 in that then-divided city to repudiate communism and famously declare "Ich bin ein Berliner," German for "I am a Berliner." Since then, presidents from Ronald Reagan to Bill Clinton have used Berlin speeches to articulate broad themes about freedom and international alliances.

Obama, fresh from a two-day summit of the Group of Eight industrial economies, placed his hand over his heart outside the sunny presidential palace as a German military band played "The Star-Spangled Banner," the American national anthem. He and German President Joachim Gauck inspected a lineup of German military troops before entering the palace, stopping to greet children who waved American and German flags.

The high point for Obama on Wednesday will be a speech at the Brandenburg Gate, a symbol of Germany's division and later reunification. It is a venue that Merkel denied him in 2008, saying only sitting presidents were granted such an honor. Obama's speech will also mark the first time a U.S. president will speak from the east side of the former Wall, a symbolic crossing into territory formerly under Soviet control.

The past context ? and the weight of it ? are not lost on the White House.

"This is a place where U.S. presidents have gone to talk about the role of the free world essentially," said Obama's deputy national security adviser, Ben Rhodes. "He is seeking to summon the energy and legacy of what's been done in the past and apply it to the issues that we face today."

Rhodes said Obama will make the case that even though the Berlin Wall came down 23 years ago and the threat of nuclear war has dissipated, the type of activism apparent during the Cold War needs to be applied to such current challenges as nuclear arms control and non-proliferation, climate change, and the push for democratic values beyond the United States and Europe.

The president has previously called for reductions to the stockpiles and is not expected to outline a timeline for this renewed push. Also in Wednesday's speech, Obama will press Congress to pass a nuclear test ban treaty, seeking to revive an effort that has stalled in recent years.

The visit was attracting widespread attention in Germany. People waved and snapped photos as Obama sped by after his arrival and a thick cluster awaited the motorcade as it passed the Brandenburg Gate. An evening news show in Berlin devoted itself to the president's visit, highlighting "Das Biest," or "The Beast," as the president's armored limousine is called.

There have been a few small protests, including one directed against the National Security Agency's surveillance of foreign communications, where about 50 people waved placards taunting, "Yes, we scan."

Merkel has said she was surprised at the scope of the spying that was revealed and said the U.S. must clarify what information is monitored. But she also said U.S. intelligence was key to foiling a large-scale terror plot and acknowledged her country is "dependent" on cooperating with American spy services.

For Merkel, the visit presents an opportunity to bolster her domestic standing ahead of a general election in September.

The U.S. and the Germans have clashed on economic issues, with Obama pressing for Europe to prime the economy with government stimulus measures, while Merkel has insisted on pressing debt-ridden countries to stabilize their fiscal situations first.

But the two sides have found common ground on a trans-Atlantic trade pact between the European Union and the U.S. At the just-completed G-8 summit, the leaders agreed to hold the first talks next month in the U.S.

___

Associated Press writers Julie Pace, Robert Reid and Frank Jordans contributed to this report.

Associated Press

Source: http://hosted2.ap.org/APDEFAULT/3d281c11a96b4ad082fe88aa0db04305/Article_2013-06-19-Obama/id-63df40c6459542d7919ce77d588c0a87

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Beliefs about causes of obesity may impact weight, eating behavior

June 18, 2013 ? Whether a person believes obesity is caused by overeating or by a lack of exercise predicts his or her actual body mass, according to new research published in Psychological Science, a journal of the Association for Psychological Science.

Obesity has become a pressing public health issue in recent years, with two-thirds of U.S. adults classified as overweight or obese and similar trends unfolding in many developed nations. Researchers Brent McFerran of the Ross School of Business at the University of Michigan and Anirban Mukhopadhyay of Hong Kong University of Science and Technology wondered whether individual beliefs might play a role in these trends.

From an initial online survey, they discovered that people seem to subscribe to one of two major beliefs about the primary cause of obesity:

"There was a clear demarcation," says McFerran. "Some people overwhelmingly implicated poor diet, and a roughly equal number implicated lack of exercise. Genetics, to our surprise, was a far distant third."

McFerran and Mukhopadhyay wanted to dig deeper to see if the pattern could be replicated and, if so, what implications it might have for behavior. They conducted a series of studies across five countries on three continents.

Data from participants in Korea, the United States, and France showed the same overall pattern: Not only did people tend to implicate diet or exercise as the leading cause of obesity, people who implicated diet as the primary cause of obesity actually had lower BMIs than those who implicated lack of exercise.

"What surprised me the most was the fact that we found lay theories to have an effect on BMI over and above other known factors, such as socio-economic status, age, education, various medical conditions, and sleep habits," says McFerran.

The researchers hypothesized that the link between people's beliefs and their BMI might have to do with how much they eat.

A study with Canadian participants revealed that participants who linked obesity to lack of exercise ate significantly more chocolates than those who linked obesity to diet. And a study with participants in Hong Kong showed that participants who were primed to think about the importance of exercise ate more chocolate than those primed to contemplate diet.

These findings provide evidence that our everyday beliefs about obesity may actually influence our eating habits -- and our body mass.

According to Mukhopadhyay, this is "the first research that has drawn a link between people's beliefs and the obesity crisis, which is growing as fast as people's waistlines are."

The new findings suggest that, in order to be effective, public health campaigns may need to target people's beliefs just as much as they target their behaviors.

This research was supported by a grant from the Social Sciences and Humanities Research Council of Canada and Hong Kong Research Grants Council Grant CERG 642810.

Source: http://feeds.sciencedaily.com/~r/sciencedaily/top_news/~3/ePMDwKSNS64/130618113858.htm

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GAFFE: Biden botches name of congresswoman fighting cancer!

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Vice President Joe Biden confused a congresswoman fighting lung cancer during a speech on guns yesterday.

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Biden mistook Rep. Carolyn McCarthy (D-L.I.). with Rep. Carolyn Maloney (D-Manhattan) while praising McCarthy and others for ?not giving up? on pushing tougher gun laws.

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?Nor has Carolyn Maloney who is one of the leading advocates for rational gun policy and is not here today,? Biden said.

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?The Congresswoman is not here today because she is fighting another fight. She?s fighting a fight that we have her in our prayers. She?s fighting lung cancer. She?ll beat that, just like she is gonna come back and help beat this gun lobby that prevented any kind of rational action from happening.? He added.

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New York Democratic Rep. Carolyn McCarthy is fighting lung cancer and is taking a leave from congress for treatment.

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Biden:?We have not given up. Janet Napolitano has not given up, Eric Holder has not given up, Richard Blumenthal has not given up, Congressman Eliot Engel has not given up, Congressman Mike Thompson has not given up and Speaker Brendan Sharkey of the Connecticut House has not given up. I just wish we had the success you had up in Connecticut with your governor and with your legislature. And Mary Pam O?Connor of Santa Monica hasn?t given up, nor has Carolyn Maloney, who is one of the leading advocates for rational gun policy, who is not here today because the congresswoman is fighting another fight. She is fighting a fight that, we have her in our prayers, she is fighting lung cancer. She will beat that. Just like she is going to come back and help us beat this gun lobby that has prevented the rational action from happening.?

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Source: http://nation.foxnews.com/2013/06/19/gaffe-biden-botches-name-congresswoman-fighting-cancer

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Tuesday, June 18, 2013

Obama opens 24-hour trip to Germany

(AP) ? President Barack Obama is opening a 24-hour visit to Germany, the culmination of which will be a speech Wednesday at Berlin's iconic Brandenburg Gate.

Obama will also hold meetings with German Chancellor Angela Merkel and other government officials. He arrived in Berlin following a two-day summit of the Group of 8 industrial nations in Northern Ireland.

The president's visit comes nearly 50 years to the day after John F. Kennedy's famous Cold War address in Berlin.

Obama's trip is sure to draw comparisons to his 2008 visit to the once-divided city as a candidate for the White House. He received a rock star welcome, with 200,000 people gathering to hear him deliver remarks at Berlin's Victory Column.

Associated Press

Source: http://hosted2.ap.org/APDEFAULT/89ae8247abe8493fae24405546e9a1aa/Article_2013-06-18-EU-Obama-Germany/id-9e66d307ec7f4a2db12c27dd569d1288

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EasyJet to expand fleet with Airbus A320, A320neo aircraft

LONDON (Reuters) - British budget airline EasyJet Plc said on Tuesday it would expand its fleet with more Airbus jets, with an order for 35 current generation A320 aircraft and 100 new generation A320neo.

The A320 aircraft would be delivered between 2015 and 2017 under an existing agreement, it said, and the next generation aircraft would be delivered between 2017 and 2022 under a new deal.

It said it had also agreed rights to buy up to a further 100 A320neo family aircraft.

Chief executive Carolyn McCall said both Airbus and Boeing competed hard for the easyJet business.

"Ultimately, Airbus offered us the best deal, and at a price with a greater discount to the list price than their landmark fleet purchase with easyJet in 2002," she said.

(Reporting by Paul Sandle; Editing by Neil Maidment)

Source: http://news.yahoo.com/easyjet-expand-fleet-airbus-a320-a320neo-aircraft-062546799.html

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Tuesday, June 11, 2013

Dispatch audio reveals details of Paris Jackson suicide attempt

LOS ANGELES (Reuters) - Paris Jackson, the 15-year-old daughter of late pop star Michael Jackson, swallowed 20 pain pills and cut her arm last week in an apparent suicide attempt, according to emergency dispatch audio released on Tuesday.

The audio released by the Los Angeles County Fire Department casts new light on the June 5 incident at the Jackson's family residence in Calabasas, California, which prompted a judge who oversees her guardianship case to order an investigation into her "health, education and welfare."

The call between emergency dispatchers indicated that Paris took 20 Motrin tablets, an over-the-counter pain medication, and cut her arm with a kitchen knife.

The minute-long audio clip also described the teenager as awake and breathing before being rushed to a local hospital.

Paris and her two brothers Prince Michael and Prince Michael II, also known as Blanket, live under the court-ordered custody of their 83-year-old grandmother, Katherine Jackson, and cousin, T.J. Jackson, the son of Jackson's older brother Tito.

Michael Jackson died in 2009 at age 50 from a lethal dose of the surgical anesthetic propofol.

Los Angeles Superior Court Judge Mitchell Beckloff ordered an investigator to "address the status of the minor child, Paris Michael Katherine Jackson, and recent media reports concerning her welfare," according to court documents filed after the she entered a hospital last week.

Katherine Jackson's attorney, Perry Sanders, said last week that Paris was "physically fine."

Sanders' office did not immediately respond to a request for comment.

Jackson has recently rekindled her relationship with her biological mother, Debbie Rowe, who was married to Michael Jackson from 1996 to 1999 and turned over custody of her two children with him as part of their divorce.

(Reporting by Eric Kelsey; editing by Patricia Reaney and Christopher Wilson)

Source: http://news.yahoo.com/dispatch-audio-reveals-details-paris-jackson-suicide-attempt-224246480.html

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Levees, removable walls in plan to protect NYC

Mayor Michael Bloomberg speaks at the Real Estate Board of New York on Thursday, May 30, 2013, in New York. Two threatening letters containing traces of the deadly poison ricin were sent to Bloomberg in New York and his gun-control group in Washington, police said. The anonymous letters were opened in New York on Friday at the city's mail facility in Manhattan and in Washington on Sunday at an office used by Mayors Against Illegal Guns, the nonprofit started by Bloomberg, police said Wednesday. (AP Photo/John Minchillo)

Mayor Michael Bloomberg speaks at the Real Estate Board of New York on Thursday, May 30, 2013, in New York. Two threatening letters containing traces of the deadly poison ricin were sent to Bloomberg in New York and his gun-control group in Washington, police said. The anonymous letters were opened in New York on Friday at the city's mail facility in Manhattan and in Washington on Sunday at an office used by Mayors Against Illegal Guns, the nonprofit started by Bloomberg, police said Wednesday. (AP Photo/John Minchillo)

NEW YORK (AP) ? Removable flood walls would be set up for much of lower Manhattan, a 15-to-20-foot levee would guard part of Staten Island and gates and levees would shield Brooklyn as part of a nearly $20 billion plan Mayor Michael Bloomberg proposed Tuesday to protect New York City from storms and the effects of global warming.

Bloomberg's proposals also include building dunes in Staten Island and the Rockaways, firming up the shoreline with bulkheads in various neighborhoods and considering building a levee and a new "Seaport City" development at the South Street Seaport that would echo nearby Battery Park City.

The mayor also is suggesting giving $1.2 billion in grants to property owners to flood-proof their buildings and $50 million to nursing homes to improve theirs; making hospitals even in rarely flooded areas upgrade their pumps and electrical equipment; and expanding beaches and marshes, among other ideas.

"This plan is incredibly ambitious," Bloomberg said in a speech at the Brooklyn Navy Yard, acknowledging that much of the work would extend beyond the end of his term this year. "This is urgent work, and it must begin now.

"Piece by piece, over many years and even decades, we can build a city that's capable of preparing better, withstanding more and overcoming anything."

Fueled by the city's blow from Superstorm Sandy last fall, the sweeping proposals represent a sizeable step up in scale and urgency for a mayor who has for years emphasized the threat climate change poses to the nation's biggest city, which has 520 miles of coastline.

It remains to be seen how the ideas will fare in a future mayoral administration, and what kind of support ? financial and otherwise ? they might ultimately get from the federal government and other entities that would be involved, not to mention from New Yorkers themselves. Bloomberg acknowledged that some of the ideas could block views and otherwise prove controversial, but "if we're going to save lives and protect the lives of communities, we're going to have to live with some new realities," he said.

Bloomberg said the city and federal money already allocated for Sandy relief would provide $10 billion for the projects, and the city believed it could get at least an additional $5 billion in federal money.

The recommendations draw from updated predictions from the New York City Panel on Climate Change, a scientists' group convened by the city.

The average day could be 4 degrees to nearly 7 degrees hotter by mid-century, the panel estimates. A once-in-a-century storm would likely spur a surge 5 or more feet higher than did Sandy, which sent a record 14-foot storm tide gushing into lower Manhattan.

And with local waters a foot to 2? feet higher than they are today, 8 percent of the city's coastline could see flooding just from high tides, the group estimates. Most of that coast is in a relatively undeveloped area near a bay.

City Hall, the state government and others have released warnings over the years about climate risks. The city has required some new developments in flood zones to be elevated and has restored wetlands as natural barriers, among other steps.

"Sandy, obviously, increased the urgency of dealing with this and the need to plan and start to take concrete steps," Deputy Mayor Caswell Holloway said Monday.

The new projections echo 2009 estimates from the climate change panel, but the timeframe for some upper-end possibilities has moved up from the 2080s to mid-century.

"The overall numbers are similar, but we have more compelling evidence now that (a more severe scenario from 2009) is looking like a more realistic possibility now," due to improved computer models and more evidence that some ice sheets are melting, said Radley Horton, a climate scientist with Columbia University's Earth Institute and a researcher with the city climate panel.

Scientists have reached a consensus on global warming but still debate how severe the effects will be.

___

Follow Jennifer Peltz at http://twitter.com/jennpeltz

Associated Press

Source: http://hosted2.ap.org/APDEFAULT/b2f0ca3a594644ee9e50a8ec4ce2d6de/Article_2013-06-11-Bloomberg-Climate%20Change/id-da073a95d7ae470ba6473df09e0a806d

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Olympus Tough TG-2 iHS


When we reviewed the Olympus Tough TG-1 iHS last year we found it to be one of the best rugged cameras on the market. It was a compact shooter with a fast lens that could shoot deep underwater, and survive drops, pressure, and extreme temperatures?and it took great photos in all types of light. It wasn't perfect; while the lens was sharp in the center, its edge performance was disappointing, and the lens made some noise that was audible when recording movies. The Tough TG-2 iHS ($379.99 direct) is, at its heart, the same 12-megapixel camera with a few modest upgrades and a lower asking price. We haven't seen another rugged camera that could challenge the TG-1, which makes the TG-2 an easy Editors' Choice award winner.

Design and Features
The TG-2 forgoes the standard rugged point-and-shoot design, which places the lens in the top corner. In addition to making it look a bit more like a standard camera, it has the unique ability in its class to accept 40.5mm filters and conversion lenses?although you will need to grab the $20 CLA-T01 adapter to support filters. The camera itself measures 2.6 by 4.5 by 1.1 inches (HWD) and is a bit heavy at 8.1 ounces. The only other rugged camera series we've seen with a centered lens is from Pentax; its latest WG-3 GPS weighs the same, but it's a bit bigger at 2.5 by 4.9 by 1.3 inches. The TG-2 is available in versions?one is red with black accents, and the other (which we reviewed) is black with red accents.

A 4x zoom lens covers a nice 25-100mm (35mm equivalent) field of view, which is a bit wider than the 28-140mm lens built into the?Canon Power Shot D20. More impressive is its maximum f/2 aperture, which lets in about four times as much light as the D20's f/3.9 lens at its widest angle. The only other rugged cameras in this class that match the TG-2's lens in terms of light gathering are the Pentax WG-3 GPS and WG3.

The f/2 design makes it possible to shoot at faster shutter speeds without pumping up the ISO, and the wide 25mm field of view is ideal for underwater use, as refraction makes objects appear a bit closer than they actually are. If you need to shoot wider you can add a fisheye conversion lens; it sells for $140, and that includes the adapter ring necessary to mount it. There's also a teleconverter available for $130 with the adapter ring; it multiplies the effective focal length of by 1.7x, delivering a 43-170mm field of view. Both accessories can be used underwater.

The 3-inch rear display uses OLED technology rather than the more-common LCD. Even though it only has a 610k-dot resolution, most cameras in this class, including the Olympus Tough TG-830 iHS, use LCD displays with 460k dots. The OLED display is a bit punchier thanks to enhanced contrast, and it's bright enough for outdoor use, even on bright days.

The bulk of the camera's controls are to the right of the display. These include a zoom rocker, video recording button, and a four-way controller. The TG-2 also sports a Mode dial to change shooting settings?an amenity that is often excluded from tough cameras. The controls aren't perfect?you'll still have to dive into an overlay menu to adjust ISO, exposure compensation, drive mode, and some other common functions, but if you're the type of shooter who likes to use art filters or scene modes, having them readily available via the physical dial is a benefit. The camera lacks full manual control and shutter priority modes, but it does support aperture priority.

The camera is rated for underwater use up to 50 feet, which is the best that we've seen in a tough camera. It can also survive drops from heights up to 7 feet, can withstand 220 pounds of pressure, and operate in temperatures as low as 14?F. If 50 feet isn't deep enough, you can add a dedicated underwater housing to the camera. At $310 it costs nearly as much as the camera itself, but will allow you to dive and shoot all the way down to 145 feet. If your needs for a rugged camera aren't this extreme, the?Panasonic Lumix DMC-TS20 is a great bargain, although it can only hit depths of 16 feet, is outfitted with a fairly low-resolution LCD, and lacks most of the bells and whistles found on the TG-1.

In addition to a GPS, which only takes about 50 seconds to lock onto a signal, the camera has an electronic compass and a manometer. The former will point you north, and can also act as a multi-axis level to help ensure that you are holding the camera parallel to the horizon. The latter records elevation and underwater depth, and will warn you as you approach the 50-foot underwater limit.

Rounding out the camera's feature set is an LED light, positioned on the front of the camera. This serves two purposes?it speeds up the autofocus system, and can be used to add light to your subject when shooting in Macro mode. It doesn't work as well as the six-LED setup on the Pentax WG-3 GPS, but certainly comes in handy when shooting in macro mode.

Performance and ConclusionsOlympus Tough TG-2 iHS : Benchmark Tests
The TG-2 is fast. It starts and fires a shot in about 0.9-second, records a 0.1-second shutter lag, and can fire off full-resolution photos continuously at 5.3 frames per second. It's rated to keep going for 25 shots, but we managed to rattle off more than 50 without any slowdown when using a SanDisk 95MBps memory card. There are also a couple high-speed shooting modes that drop the resolution to 3 megapixels but let you grab up to 100 images at 15 or 60 frames per second. There aren't many rugged compacts that can keep up with the TG-2; of current models we've tested, the Olympus TG-830 comes closest. It starts in 1.4 seconds, can capture a burst of 16 shots at 10 frames per second, can shoot continuously at 2.5 frames per second, and its shutter lag is a respectable 0.2-second.

Like the TG-1, the TG-2's image sharpness is its weakest aspect. We tested it using?Imatest, which shows that at 25mm f/2 it only manages 1,673 lines per picture height using a center-weighted metric, shy of the 1,800 lines we use to deem an image acceptably sharp. The center sharpness is quite good, nearly 2,100 lines, but the mid-center and edge scores bring the overall rating down. You can set the camera to aperture priority and stop down to f/2.8, which we recommend doing when the light allows it. At f/2.8 the lens notched 1,795 lines; the edges were still soft, but the mid-center and center scores were high enough to bring the average up. The Pentax WG-3, which also has an f/2 lens design, does better; it manages 1,948 lines at 25mm f/2.

Imatest also measures noise, which shows up at higher ISO settings and can hurt image detail and add an unwanted graininess to photos. The TG-2 keeps noise under 1.5 percent through ISO 1600, which is impressive for a compact camera with a 1/2.3-inch sensor. Some detail is smudged at this setting, but images are still useable. The Canon PowerShot D20 is the closest we've seen to the TG-2 in terms of image quality and noise control at high ISO settings; but it only keeps noise below 1.5 percent through ISO 800.

Video is recorded in QuickTime format at 1080p60 or 720p60 quality. The footage is crisp and smooth, but the audio is another story. The sound of the lens zooming or focusing is very loud on the soundtrack. Hidden under a double-locking door are two connectors: one micro HDMI and one proprietary USB. The former lets you connect the camera to an HDTV, and the latter will let you plug it into a computer or into an outlet (using the included AC adapter) for battery charging. There's no dedicated battery charger included, so you'll have to charge in-camera unless you spend an extra $60 on the Olympus UC-90 charger. Standard SD, SDHC, and SDXC cards are supported; the card slot and the battery are in the same compartment, also protected by a double-locking door.

The TG-2 iHS is the best rugged camera that we've seen, and it's worth your money unless you already own a TG-1. Other than the increased depth rating and the addition of an aperture priority mode, the cameras are basically the same. This isn't a bad thing; the TG-1 is an excellent camera, and the TG-2 delivers the same performance at a slightly lower asking price. If $380 is too salty for your wallet, there are some good alternatives available for less money. The Olympus Tough TG-830 iHS is priced at $280 and is also an impressive performer, it just lacks a fast lens. There's also the Pentax WG-3 sells for about $300; it has a fast lens, but doesn't do as well at high ISO settings and lacks a GPS. But if you are in the market for a rugged camera, and you can afford it, the TG-2 is the way to go.

Source: http://feedproxy.google.com/~r/ziffdavis/pcmag/~3/ks85ofLwNZc/0,2817,2420057,00.asp

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Monday, June 10, 2013

PLA / ABS and Pets - RepRap Forums

Hi all!

I'm just starting to get into the printing scene (still haven't received my first printer, the waiting is getting very old very fast), when it occurred to me that I may need to be careful if I ever get a bird like I've been thinking about. I searched around and had little luck, so I thought I'd ask here if any of you have seen or heard anything useful.

Obviously I would never expose a bird to ABS fumes. That's just stupid, as it's a recipe for a dead bird and a lot of tears.

My questions are:

    [1] Is printing PLA without a fume collection device typically considered safe around pets?
    [2] Should a pet get a hold of something I've printed, either out of PLA or ABS, and chewed it, would just chewing it be harmful?
    [3] Same as above, but including ingestion of the chewed bits?
    [4] If I had to choose ABS or PLA, would a pet getting a hold of something made of one of those be worse than the other?
    [5] If I can typically consider one of these plastics safe for a pet, could I reasonably make objects designed for a pet to play around with? Certain parrots like puzzles, and imo it would be cool to design progressively harder puzzles to solve.
Thanks!

Source: http://forums.reprap.org/read.php?1,216657,216657

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Based on earlier successes, NIH awards new study in cancer research to Virginia Tech's Chang Lu

Based on earlier successes, NIH awards new study in cancer research to Virginia Tech's Chang Lu [ Back to EurekAlert! ] Public release date: 10-Jun-2013
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Contact: Lynn Nystrom
tansy@vt.edu
540-231-4371
Virginia Tech

Progress made in the technology development for studying protein-DNA interactions, conducted by Chang Lu, associate professor of chemical engineering and a core faculty member of the School of Biomedical Engineering and Sciences at Virginia Tech, has led to the National Institutes of Health (NIH) awarding a new project to continue his groundbreaking work.

Working with Albert Baldwin, a cancer biologist of the University of North Carolina at Chapel Hill, they are advancing the development of a specific technology that Lu said could "revolutionize the study of molecular mechanisms involved in cancer development in multiple aspects."

Lu and Baldwin are using an investigative procedure called chromatin immunoprecipitation (ChIP) to examine the protein binding to DNA sites that can lead to cancer.

This ChIP assay is not new. In the past, its drawback was the number of cells needed for each individual test more than one million cells. "This large sample practically prevents the use of ChIP on samples from animal models and patients," Lu explained.

So, Lu and Baldwin are developing a more specialized ChIP assay conducted on microchip devices for studies based on tiny amounts of primary cell samples from mice and humans. Lu's preliminary work showed they could produce reliable results based on as few as 50 cells as opposed to the need for one million cells. This work was previously described in the journal, Lab on a Chip.

With their new test that provides "an ultrahigh sensitivity," they believe they can design ChIP assays that will be able to study tumor-initiating cells and monitor the dynamics at the molecular level in how the tumor cells multiply, possibly leading to the spread of cancer.

Lu is known for making significant contributions to the practice of cellular manipulation and analysis. His research group at Virginia Tech has demonstrated a variety of new technologies ranging from electroporation for gene delivery to analysis of single cells.

His new NIH grant, awarded over three years at a total amount of $710,000, is part of the institutes' National Cancer Initiative that focuses on early-stage innovative molecular analysis technology (IMAT) development for cancer research (http://imat.cancer.gov/). The new work was also enabled by seed grants from the Virginia Tech Institute for Critical Technology and Applied Science (ICTAS) NanoBio Thrust area (http://www.ictas.vt.edu/research/nanobio.php).

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AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert! system.


Based on earlier successes, NIH awards new study in cancer research to Virginia Tech's Chang Lu [ Back to EurekAlert! ] Public release date: 10-Jun-2013
[ | E-mail | Share Share ]

Contact: Lynn Nystrom
tansy@vt.edu
540-231-4371
Virginia Tech

Progress made in the technology development for studying protein-DNA interactions, conducted by Chang Lu, associate professor of chemical engineering and a core faculty member of the School of Biomedical Engineering and Sciences at Virginia Tech, has led to the National Institutes of Health (NIH) awarding a new project to continue his groundbreaking work.

Working with Albert Baldwin, a cancer biologist of the University of North Carolina at Chapel Hill, they are advancing the development of a specific technology that Lu said could "revolutionize the study of molecular mechanisms involved in cancer development in multiple aspects."

Lu and Baldwin are using an investigative procedure called chromatin immunoprecipitation (ChIP) to examine the protein binding to DNA sites that can lead to cancer.

This ChIP assay is not new. In the past, its drawback was the number of cells needed for each individual test more than one million cells. "This large sample practically prevents the use of ChIP on samples from animal models and patients," Lu explained.

So, Lu and Baldwin are developing a more specialized ChIP assay conducted on microchip devices for studies based on tiny amounts of primary cell samples from mice and humans. Lu's preliminary work showed they could produce reliable results based on as few as 50 cells as opposed to the need for one million cells. This work was previously described in the journal, Lab on a Chip.

With their new test that provides "an ultrahigh sensitivity," they believe they can design ChIP assays that will be able to study tumor-initiating cells and monitor the dynamics at the molecular level in how the tumor cells multiply, possibly leading to the spread of cancer.

Lu is known for making significant contributions to the practice of cellular manipulation and analysis. His research group at Virginia Tech has demonstrated a variety of new technologies ranging from electroporation for gene delivery to analysis of single cells.

His new NIH grant, awarded over three years at a total amount of $710,000, is part of the institutes' National Cancer Initiative that focuses on early-stage innovative molecular analysis technology (IMAT) development for cancer research (http://imat.cancer.gov/). The new work was also enabled by seed grants from the Virginia Tech Institute for Critical Technology and Applied Science (ICTAS) NanoBio Thrust area (http://www.ictas.vt.edu/research/nanobio.php).

###


[ Back to EurekAlert! ] [ | E-mail | Share Share ]

?


AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert! system.


Source: http://www.eurekalert.org/pub_releases/2013-06/vt-boe061013.php

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94% Room 237

All Critics (117) | Top Critics (31) | Fresh (111) | Rotten (7) | DVD (1)

The human brain is a marvellously suggestible organ.

[A] strange, frustrating, occasionally fascinating doc ...

"Room 237" evolves from an ode to movie love at its most delirious to a wry examination of the crackpot mind at work.

There's enough real evidence supporting the theory that Kubrick was a genius, and that's pretty entertaining all by itself.

It's about the human need for stuff to make sense - especially overpowering emotional experiences - and the tendency for some people to take that sense-making to extremes.

The results can range from enlightening - Kubrick did like to mess with things - to embarrassing. But it's never dull. "Room 237" shines.

It has the same entertainment value as listening to a late-night radio host indulge his listeners on Roswell, Area 51 and 9/11. Everything sounds completely crackers, until it all makes crazy sense.

What emerges from Room 237 is not a denigration of conspiracies, but a kind of celebration of our ability to create patterns where (perhaps) none exist.

"Room 237" could become an essential companion piece to "The Shining" from now on. For those who see both, it will be impossible to think about one without the other.

...all about the work of criticism - finding fresh avenues of delight.

Watching it makes you feel like you're attending a really entertaining film class where your classmates confidently let their freak flags fly.

It's an essay about the human need to reject the notion of a random universe and find order and meaning in existence. These people are developing their own creation myths, with Kubrick the mastermind responsible for the Intelligent Design.

Termitic film nerds could chow down for years on the wood chips.

You know when "Room 237? starts getting really scary? When the people in the film start making sense.

Kubrick fans and movie geeks will want to check this film out as soon as possible

Kubrick fans will take 'Shining' to 'Room 237.'

The credibility of these theories ranges from faintly plausible to frankly ridiculous, but Ascher isn't interested in judging them; his movie is more about the joys of deconstruction and the special kind of obsession that movies can inspire.

Some of the interpretations seem more of a stretch than others but all are entertainingly presented by director Rodney Ascher. (The movie) serves as a testament to Stanley Kubrick's cinematic mastery.

As fascinating as it is frustrating

It is nice to see a doc that makes you smile instead of making you angry. Anyone who is a fan of Stanley Kubrick will eat this up.

Powered by a deep and abiding affection for both The Shining and Kubrick in general, Room 237 is an amuse-bouche of remix culture.

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Source: http://www.rottentomatoes.com/m/room_237_2012/

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